Coding
Part:BBa_K1746001:Design
Designed by: Christopher Lastihenos Group: iGEM15_Cooper_Union (2015-09-11)
TdT delta26-143 (Truncated TdT Variant)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1032
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 581
Design Notes
This DNA sequence was based on a truncated variant of bovine TdT which was then codon optimized for optimal expression in E. Coli, and chemically synthesized.
Source
This DNA sequence was chemically synthesized and codon optimized for expression in E. Coli.